胚胎/IPS干細(xì)胞無血清培養(yǎng)基產(chǎn)品信息: NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, with HSA 貨號:05-100-1A/B,人類胚胎干細(xì)胞無血清培養(yǎng)基(含人血清白蛋白) AF NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, without HSA 貨號: 05-102-1A/B,人類胚胎干細(xì)胞無血清培養(yǎng)基(不含人血清白蛋白)
人類胚胎干細(xì)胞研究是當(dāng)前zui熱門的科研課題之一,經(jīng)由無限制的增殖及適當(dāng)?shù)恼T導(dǎo)分化后,胚胎細(xì)胞干細(xì)胞可分化成人體中各種類型的細(xì)胞,如神經(jīng)細(xì)胞、心肌細(xì)胞及軟骨細(xì)胞,這些特點(diǎn)造就胚胎干細(xì)胞未來在人類醫(yī)學(xué)健康應(yīng)用上不可估量的前景。 傳統(tǒng)干細(xì)胞培養(yǎng)過程中必需添加的異種動物(非人類)成分,如牛血清或豬血清,使之無法*排除人畜共染病毒、朊病毒(Prion)及支原體污染細(xì)胞 的可能性,這是造成人類干細(xì)胞進(jìn)入臨床應(yīng)用的主要障礙之一。經(jīng)由使用成分明確、全合成的無血清干細(xì)胞培養(yǎng)基可有效避免人畜共染疾病的問題,同時全合成培養(yǎng) 基無批間差異,培養(yǎng)條件容易保持*,實(shí)驗(yàn)的重復(fù)性大為提高。應(yīng)用無血清干細(xì)胞培養(yǎng)基可為干細(xì)胞在心血管疾病、神經(jīng)性退化及癌癥的臨床治療方面打開了一扇 大門。 為了滿足科研與生物醫(yī)藥企業(yè)的需求,BI公司與以色列國家科學(xué)研究院(Technion-Israel Institute of Technology)的Dr. Itskovitz共同合作開發(fā)了一代、擁有自主知識產(chǎn)權(quán)的人類胚胎干細(xì)胞(hESC)和誘導(dǎo)胚胎干細(xì)胞(iPSC)無血清*培養(yǎng)基—— NutriStem®。 Dr. Joseph Itskovitz己在Stem Cells, Stem Cell Dev, PNAS, Nature及Science等雜志發(fā)表近百篇文章,并有三項(xiàng)胚胎干細(xì)胞培養(yǎng)與分化誘導(dǎo),同時也是1998年*篇分離人類胚胎干細(xì)胞論文的共同作者,現(xiàn)任職于以色列國家科學(xué)研究院Rambam Medical Center。美國國家衛(wèi)生研究院干細(xì)胞庫中批準(zhǔn)用于科研的22株人類胚胎干細(xì)胞由其團(tuán)隊(duì)提供了8株。 Embryonic stem cell lines derived from human blastocysts. J. A. Thomson, J. Itskovitz-Eldor, et. al., Science. Vol. 282, no. 5391, 1145-1147. 1998. NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, with HSA 貨號:05-100-1A/B,人類胚胎干細(xì)胞無血清培養(yǎng)基(含人血清白蛋白) 開瓶即可使用的*培養(yǎng)基,不含異源動物成分,添加了醫(yī)療級的人血清白蛋白(HSA:Human Serum Albumin),以適用于無滋養(yǎng)層培養(yǎng)法(Feeder-Free),如Matrigel™或conditioned medium培養(yǎng)。 AF NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, without HSA 貨號: 05-102-1A/B,人類胚胎干細(xì)胞無血清培養(yǎng)基(不含人血清白蛋白) 開瓶即可使用的*培養(yǎng)基,不含任何動物來源成分,適用于鼠胚胎纖維原細(xì)胞(Mouse Embryonic Fibroblasts-MEF)及人包皮纖維原細(xì)胞(Human Foreskin Fibroblasts-HFF)滋養(yǎng)層培養(yǎng)法。 NutriStem®已發(fā)表參考文獻(xiàn): 1. Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells. Sugii et al. PNAS. Vol. 107, No 8, 3558-3563, 2009. 2. Current technology for the derivation of pluripotent stem cell lines from human embryos. Hasegawa et al., Cell Stem Cell. Vol. 6, 521-531, 2010. 3. Progress and challenges in optimiztion of human pluripotent stem cell culture. Ge et al., Current Stem Cell research & Therapy. Vol. 5, 207-214, 2010. 4. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Warren et al., Cell Stem Cell. Vol. 7, 1-13, 2010. 5. Fibrin microbeads (FMB) loaded with mesenchymal cells support their long term survival while sealed at room temperature. Gorodetsky et al. Tissue Engineering Part C: Methods. Online Ahead of Editing, March 2011. 6. Site-specific gene correction of a point mutation in human iPS cells derived from an adult patient with sickle cell disease. Zou, et al. Blood. 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