熱烈祝賀華雅生物成為TAKARA Cellartis®干細(xì)胞研究產(chǎn)品全國代理商 作為一款創(chuàng)新型的產(chǎn)品,Cellartis DEF-CS培養(yǎng)系統(tǒng)使得單細(xì)胞干細(xì)胞操作成為常規(guī)的操作。該產(chǎn)品是專門針對人類誘導(dǎo)干細(xì)胞(hiPS)和人類胚胎干細(xì)胞(hES)研發(fā)的高效率增殖用培養(yǎng)基產(chǎn)品體系。該產(chǎn)品系統(tǒng)為all-in-one型,包括了所有組分,客戶不需要另外求購組分。該產(chǎn)品是成分確定的培養(yǎng)基產(chǎn)品,而且不需要飼養(yǎng)層。DEF-CS既可以實現(xiàn)單細(xì)胞培養(yǎng),也可以用于傳統(tǒng)的iPS培養(yǎng)模式以及大規(guī)模干細(xì)胞增殖。在使用DEF-CS培養(yǎng)基增殖干細(xì)胞時,幾乎沒有背景分化的問題,這使得細(xì)胞篩選不再必需。作為一款創(chuàng)新產(chǎn)品,利用DEF-CS培養(yǎng)干細(xì)胞時,可以使用酶消化法(enzymatic passaging)實現(xiàn)需要單細(xì)胞操作,這一特點十分有利于高通量細(xì)胞鑒別篩選(high-throughput screening)、轉(zhuǎn)染(transfection)、框架接種(scaffold seeding)等。 ■ 產(chǎn)品特點 | · Feeder-free and chemically defined culture system for efficient expansion of human iPS cells · Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation · Complete kit includes 500 ml basal medium, additives, and coating compound · Robust system provides high reproducibility and stable growth · Maintains cells in an undifferentiated state with virtually no background differentiation ■ 產(chǎn)品應(yīng)用 | · Scale-up and mass production of human iPS cells · Single-cell culture of human iPS cells · Transfection and reprogramming · High-throughput screening · Tissue engineering (seeding cells on a scaffold) | | Code No. | Product | Size | Y30012 | Cellartis® DEF-CS™ 500 COAT-1 | 4 mL | Y30010 | Cellartis® DEF-CS™ 500 Culture System | 1 Kit | Y30016 | Cellartis® DEF-CS™ 500 Additives | 1 Kit |
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Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems. |
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